A PNA-based Lab-on-PCB platform for DNA quantification

Lab-on-PCB DNA diagnostic platform, exploiting peptide nucleic acid (PNA) sequences as probes was developed. The study also showcases a fully integrated Lab-on-PCB microsystem designed for rapid detection, which employs PCB-integrated sample delivery, achieving DNA quantification in the 0.1–100 pM range for 5 μL samples analyzed within 5 min under continuous flow. The demonstrated biosensor proves the capability of PCB-based DNA biosensors for high sensitivity and paves the way for their integration in Lab-on-PCB DNA diagnostic microsystems.